Composite

Part:BBa_K2449026:Design

Designed by: Malte Skovsager Andersen   Group: iGEM17_SDU-Denmark   (2017-10-23)


cenA-hlyA and secretion system with hlyB and hlyD regulated by penI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2327
    Illegal NotI site found at 627
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2179
    Illegal BamHI site found at 3389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 260
    Illegal NgoMIV site found at 633
    Illegal NgoMIV site found at 1135
    Illegal NgoMIV site found at 2219
    Illegal AgeI site found at 2315
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 680
    Illegal SapI site found at 2027
    Illegal SapI.rc site found at 763


Design Notes

Source

References